Sierra Nevada Yellow-legged Frog Environmental DNA Surveys

In 2014, Pacific Gas and Electric Company (PG&E) and the City of Santa Clara developed a Study Plan to characterize aquatic habitats and gather information on the presence, distribution, and relative abundance of special-status amphibian and aquatic reptile species at, and around, Bucks Creek Hydroelectric Project (FERC Project No. 619) reservoirs and Project-affected stream reaches. The Licensees conducted this supplementary study in 2017 to inform discussions of potential effects of Project operations on Sierra Nevada yellow-legged frog (Rana sierrae) in tributary streams to Milk Ranch Conduit.

Stillwater Sciences performed Sierra Nevada yellow-legged frog Visual Encounter Surveys (VES) and environmental DNA (eDNA) sampling along three high-gradient intermittent stream reaches. The objectives of the surveys and eDNA sampling were to determine whether any life stages of Sierra Nevada yellow-legged frogs were present and using habitats within the Project-affected reaches, and to compare the outcomes of VES and eDNA sampling methodologies.


Location: Bucks Creek Hydroelectric Project, Plumas, CA
Client: Pacific Gas & Electric Company
Project Manager: Scott Wilcox

Project Lead(s)

Scott Wilcox
email | profile

Work Products

View the gallery of imagesimage # image #
eDNA Sampling Poster